Protein_Domain
NLS

Part:BBa_M45417:Design

Designed by: Andrew Vanderwerf   Group: UtahState_BE5930_-_S14   (2015-04-10)


Nuclear Localization Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


=Design Notes

This part is a tag that will come before the protein of choice. When attaching this sequence to your protein remove the start codon from the protein and have it come immediately before your protein. No other modification of the protein should be necessary. This part allows the protein access to the nucleus of a eukaryotic cell. This part has no known function in prokaryotic cells.

Source

Synthetically created part.

References

"An optogenetic gene expression system with rapid activation and deactivation kinetics." Laura B Motta-Mena, Anna Reade, Michael J Mallory, Spencer Glantz, Orion D Weiner, Kristen W Lynch & Kevin H Gardner. Nature Chemical Biology 10, 196 - 202 (2014)